Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions

Authors

  • Hoang Huong Diem University of Science, Vietnam National University, Ha Noi; Vinmec Research Institute of Stem Cell and Gene Technology
  • Bui Thi Van Khanh University of Science, Vietnam National University, Ha Noi
  • Hoang Thi My Nhung University of Science, Vietnam National University, Ha Noi; Vinmec Research Institute of Stem Cell and Gene Technology
  • Nguyen Thanh Liem Vinmec Research Institute of Stem Cell and Gene Technology
  • Than Thi Trang Uyen* Vinmec Research Institute of Stem Cell and Gene Technology

Abstract

Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and genetic materials. In the current study, we investigated exosomes released by mature dendritic cells (mDCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) under Good Manufacturing Practice (GMP) conditions. Ultracentrifugation was used to isolate and purify the exosomes. Additionally, a transmission electron microscope (TEM) and immunoblotting were used to characterise exosomal morphology and markers. The preliminary results showed that both mDCs and UCMSCs secreted exosomes into GMP culture media. Exosomes exhibited a cup-shaped morphology and showed positive for CD63. Additionally, no difference was observed between mDC-derived exosomes and UCMSC-derived exosomes regarding marker expression or morphology. This data indicates the potential for further development of GMP exosomes for clinical application.

Keywords:

dendritic cells, exosomes, mesenchymal stem cells

DOI:

https://doi.org/10.31276/VJSTE.61(3).45-51

Classification number

3.2

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Published

2019-09-15

Received 24 June 2019; accepted 19 August 2019

How to Cite

Hoang Huong Diem, Bui Thi Van Khanh, Hoang Thi My Nhung, Nguyen Thanh Liem, & Than Thi Trang Uyen. (2019). Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions. Vietnam Journal of Science, Technology and Engineering, 61(3), 45-51. https://doi.org/10.31276/VJSTE.61(3).45-51

Issue

Section

Life Sciences