Extraction of flavonoids in pomelos’ peels using Box-Behnken response surface design and their biological activities

Authors

  • Truong Ngoc Quynh Phuong
  • Pham Van Hung
  • Nguyen Thi Lan Phi*

Abstract

The objectives of this study were to optimize the extraction conditions of flavonoids from the pomelo peel using the Box-Behnken response surface methodology and to investigate the chemical composition and biological activities of the extracts from three different pomelo species under optimized extraction conditions. Three extraction condition factors of were varied, including the solid-solvent ratio (1/15-1/45 g/ml), temperature (30-60oC), and time (20-60 min), which affected the efficiency of the total flavonoid content (TFC). The high R2 coefficient of 0.93 indicated that the experimental data obtained in this study fit well to a second-order polynomial using multiple regression analysis. The maximum TFC extracted from the pomelo peel was 6.0 mg/g (dried basis, db), which was obtained using Derringer’s desired function methodology under the following optimum conditions: a solid-solvent ratio of 1/44 g/ml, temperature of 30.7oC, and time of 34.6 min. The results also indicated that the Tan Trieu pomelo peel had the highest TFC and naringin concentration followed by the Nam Roi and Duong Hong pomelo peels, whereas the Nam Roi pomelo peel had a higher hesperidin concentration than the others. The extracts of the pomelo peels exhibited strong antioxidant activities with low IC50 values. However, these extracts showed only a slight effect on cancer cells, including lung cancer and breast cancer, when tested at 100 µg/ml.

Keywords:

antioxidant, Box-Behnken design, extraction, flavonoids, pomelo

DOI:

https://doi.org/10.31276/VJSTE.63(2).52-57

Classification number

3.1

Author Biographies

Truong Ngoc Quynh Phuong

Department of Food Technology, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Pham Van Hung

Department of Food Technology, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Nguyen Thi Lan Phi

Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Department of Food Technology, University of Technology, Vietnam National University - Ho Chi Minh City, 268 Ly Thuong Kiet Street, Ward 14, District 10 Ho Chi Minh City, Vietnam

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Published

2021-06-15

Received 9 January 2020; revised 20 March 2020; accepted 6 April 2020

How to Cite

Truong Ngoc Quynh Phuong, Pham Van Hung, & Nguyen Thi Lan Phi. (2021). Extraction of flavonoids in pomelos’ peels using Box-Behnken response surface design and their biological activities. Vietnam Journal of Science, Technology and Engineering, 63(2), 52-57. https://doi.org/10.31276/VJSTE.63(2).52-57

Issue

Section

Life Sciences