DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam
Abstract
Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives. DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L. samples collected from the mountain areas of Northern Vietnam. Based on the combined sequence data of ribosomal nuclear ITS, a part of chloroplast rbcL gene and trnL-trnF intergenic spacer, phylograms of Scrophularia L. were generated by both Bayesian inference and maximum likelihood bootstrap method. The phylogenetic analysis showed that the tested samples have a sister relationship to S. ningpoensis. Hopefully, the analysis strategy that we used would contribute to further phylogenetic analyses of medicinal plants of Vietnam in the future.
Keywords:
Bayesian inference, DNA barcodes, ITS, maximum likelihood, medicinal plants, phylogenetic, rbcL, Scrophularia L., Scrophularia LDOI:
https://doi.org/10.31276/VJSTE.60(2).56Classification number
3.3, 3.5

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Published
Received 4 December 2017; accepted 26 March 2018