Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii

Authors

  • Le Phuong Trinh* Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, Hanoi
  • Van Son Ta Faculty of Biology, University of Science, Vietnam National University, Hanoi
  • Thi Ngoc Han Luu Faculty of Biology, University of Science, Vietnam National University, Hanoi
  • Mai Phuong Nguyen Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, Hanoi
  • Thi Huong Dinh National Lung Hospital
  • Van Hung Nguyen National Lung Hospital
  • Thi Ha Doan National Lung Hospital
  • Bao Yen Pham Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, Hanoi

Abstract

This study, the presence of 4 common beta-lactamase encoding genes including OXA-23, OXA-51, VIM and IMP was determined simultaneously by multiplex PCR, either directly with sputum samples or with purified DNA, giving results within 3-4 hours instead of 2 days compared to the culture method. Within 58 samples positive with Acinetobacter baumannii collected from the hospital, OXA-51 fragment was dominant (81%), whereas, VIM fragment was only identified in one sample (DNA and sputum samples) (1.7%). The antibiograms indicated for 19 samples were used to find correlation between genotype and resistance phenotype.

Keywords:

Acinetobacter baumannii, antibiotic resistance-associated genes, beta-lactamase, multiplex PCR, susceptibility

DOI:

https://doi.org/10.31276/VJSTE.61(1).58-61

Classification number

3.5

Downloads

Published

2019-03-15

Received 11 October 2018; accepted 10 December 2018

How to Cite

Le Phuong Trinh, Van Son Ta, Thi Ngoc Han Luu, Mai Phuong Nguyen, Thi Huong Dinh, Van Hung Nguyen, Thi Ha Doan, & Bao Yen Pham. (2019). Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii. Vietnam Journal of Science, Technology and Engineering, 61(1), 58-61. https://doi.org/10.31276/VJSTE.61(1).58-61

Issue

Vol. 61 No. 1 (2019)

Section

Life Sciences

License

This work is licensed under a Attribution-NonCommercial-NoDerivatives 4.0 International

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