Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages

Authors

  • Van-Hoai Bui
  • Hong-Tham N. Vo
  • Thanh-Tuan Duong
  • Se-Kwon Kim
  • Dai-Nghiep Ngo*

Abstract

The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such as TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. The anti-inflammatory effect is mediated through the reduction of nitric oxide production and downregulation of inflammatory proteins such as inducible nitric oxide synthase (iNOS), gene expression of cytokines like TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. Target proteins were identified by western blot analysis with specific monoclonal antibodies. The levels of gene expression were determined by the RT-PCR method. The results demonstrate that GA-COS effectively reduces nitric oxide generation and downregulates iNOS protein and cytokine expression and NF-κB signalling in lipopolysaccharide (LPS) -induced RAW 264.7 cells. GA-COS exhibits significantly enhanced anti-inflammatory activity compared to the free chitooligosaccharide chain. This study lays the groundwork for future research to demonstrate that GACOS holds significant potential as a novel compound for the prevention of inflammatory diseases.

Keywords:

anti-inflammatory activity, chitooligosaccharide, chitooligosaccharide conjugated gallic acid, nitric oxide, RAW 264.7 macrophage cells

DOI:

https://doi.org/10.31276/VJSTE.66(1).96-103

Classification number

3.4, 3.5

Author Biographies

Van-Hoai Bui

Deparment of Biochemistry, Faculty of Biology-Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Ho Chi Minh City University of Industry and Trade (HUIT), 140 Le Trong Tan Street, Tay Thanh Ward, Tan Phu District, Ho Chi Minh City, Vietnam

Hong-Tham N. Vo

Deparment of Biochemistry, Faculty of Biology-Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Ho Chi Minh City University of Industry and Trade (HUIT), 140 Le Trong Tan Street, Tay Thanh Ward, Tan Phu District, Ho Chi Minh City, Vietnam

Thanh-Tuan Duong

Deparment of Biochemistry, Faculty of Biology-Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Se-Kwon Kim

Department of Marine Science and Convergence Engineering, College of Science and Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan-si, Gyeonggi-do 11558, Korea

Dai-Nghiep Ngo

Deparment of Biochemistry, Faculty of Biology-Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam

Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

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Published

2024-03-15

Received 25 May 2023; revised 4 July 2023; accepted 5 September 2023

How to Cite

Van-Hoai Bui, Hong-Tham N. Vo, Thanh-Tuan Duong, Se-Kwon Kim, & Dai-Nghiep Ngo. (2024). Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages. Vietnam Journal of Science, Technology and Engineering, 66(1), 96-103. https://doi.org/10.31276/VJSTE.66(1).96-103

Issue

Section

Life Sciences