A study on in vitro antioxidant effect of Cordyceps neovolkiana DL0004 extracts isolated in Lam Dong, Vietnam

Abstract

Cordyceps neovolkiana DL0004 is a group of insect parasitic fungi collected at an altitude of 1650 m above sea level at the top of Langbiang Mountain, Lam Dong province, Vietnam. C. neovolkiana DL0004 has been used in traditional medicine and possesses numerous biological effects such as anti-cancer, anti-inflammation, antioxidant properties. In this research, the fungi biomass and fruiting body of C. neovolkiana DL0004 were cultured and examined for their antioxidant properties using test methods such as reducing power, scavenging 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical, neutralizing 2,2’-azinobis(3-ethylbenzothiazonline-6-sulfonate (ABTS) free radical, and inhibiting xanthine oxidase (XO). The results showed that all 7 extracts of the biomass were more active than the fruiting bodies of C. neovolkiana DL0004. Furthermore, the ethyl acetate (EtOAc) extract of C. neovolkiana DL0004 biomass showed DPPH, ABTS, and XO free radical scavenging with IC₅₀ values (the concentration of the sample required to inhibit 50% of radical) of 107.01 µg/ml, 111.91 µg/ml, and 106.2248 µg/ml, respectively. Moreover, the reducing power of the EtOAc from C. neovolkiana DL0004 biomass was 0.184 at 1000 µg/ml. From the obtained results, the biomass EtOAc fraction extract of C. neovolkiana DL0004 was demonstrated to contain antioxidant compounds with potential applications in the production of functional foods.