Multiplex polymerase chain reaction (M-PCR) for bacterial vaginosis detection

Tien Sang Trieu¹*, Van Khoa Tran¹, Ha My Nguyen¹, Thi Thu Ha Nguyen¹, Quang Tung Le¹, Minh Ngoc Phung¹, Minh Quang Diep², Huong Ly Vu³

¹Vietnam Military Medical University

²Quangninh Obstetric and Pediatric Hospital

³National Obstetric Hospital

Received 11 March 2019; accepted 17 June 2019

 

Abstract:

Bacterial vaginosis (BV) is one of the most common vaginal infections in women of reproductive age. If not treated promptly, the disease can lead to serious complications affecting the fertility and long-term health of women. Research on BV requires effort from a variety of disciplines and its treatment can only be determined by coordinated actions in research and treatment. Currently, BV diagnostic methods are often based on culture techniques and Gram staining. However, molecular biology research is developing and has proved to more advantageous for identifying key pathogens. Combining both methods, we conducted a study to develop a diagnostic procedure using multiplex polymerase chain reaction (M-PCR) for simultaneous and accurate detection of the bacterial species that cause BV, incurring minimal costs and time for testing.

Keywords: bacterial vaginosis, BV, diagnosis, multiplex PCR, PCR.

Classification numbers: 3.2, 3.5