Optimization of culture conditions of iron – enriched biomass of Saccharomyces Pastorianus by Response Surface Methodology

Pham Quynh Nhu, Tran Thanh Uyen, Phan Minh Vuong, Phan Thanh Thao*

Institute of Chemical Technology, Vietnam Academy of Science and Technology (VAST)

Received 26 February 2020; accepted 20 May 2020

 

Abstract:

Yeast biomass enriched with iron is used in a profound and safe treatment for anaemia development is yeast biomass enriched with iron. In this work, response surface methodology (RSM) was used to survey the respond culture conditions (temperature, degrees brix, time and initial iron concentration) on the bioaccumulation of ferric ion (Fe³⁺) in yeast (Saccharomyces pastorianus). On the other hand, Box–Behnken design is used to determine optimum condition at 24^oC, 13^oBx of culture solid content for 49h incubation and 656ppm of initial iron concentration. Total Fe³⁺ content in biomass was significantly affected by culture temperature and degrees brix (P < 0.0001). Under optimum conditions, the maximum level of Fe (III) ions in dry cell weight of S. pastorianus was 16.82 ± 0.65 mg/g. The results from statistical analysis showed that the model was significant (P < 0.0001), and adequate.

Keywords: Saccharomyces pastorianus, Fe (III) citrate, response surface methodology, optimization, Box – Behnken.

Classification numbers: 2.2, 2.3