Comparison of the SSSperm testing kit with the Halosperm testing kit in an analysis of sperm DNA fragmentation

Main Article Content

Le Thi Quyen Nguyen Thi Minh Thu Le Thi Minh Phuong Nguyen Thi Trang

Abstract

The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration >=1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and ttn=0.97<tc=2. The two methods have similar DNA fragmentation index (DFI) results (r=0.995; p<0.001). The difference between the results of the two kits was not statistically significant (p=0.236>0.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.

DOI: https://doi.org/10.31276/VJSTE.62(2).71-76

Article Details

How to Cite
QUYEN, Le Thi et al. Comparison of the SSSperm testing kit with the Halosperm testing kit in an analysis of sperm DNA fragmentation. Vietnam Journal of Science, Technology and Engineering, [S.l.], v. 62, n. 2, p. 71-76, july 2020. ISSN 2525-2461. Available at: <https://vietnamscience.vjst.vn/index.php/VJSTE/article/view/324>. Date accessed: 11 aug. 2020.
Section
LIFE SCIENCES