Optimization of culture conditions of iron-enriched biomass of Saccharomyces pastorianus by response surface methodology

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Pham Quynh Nhu Tran Thanh Uyen Phan Minh Vuong Phan Thanh Thao

Abstract

Yeast biomass enriched with iron is used in a profound and safe treatment for anaemia. In this work, response surface methodology (RSM) was used to survey the response of culture conditions (temperature, degrees brix, time, and initial iron concentration) to the bioaccumulation of ferric ion (Fe3+) in yeast (Saccharomyces pastorianus). On the other hand, the Box-Behnken design was used to determine the optimum conditions of 24oC, 13oBx of the culture solid content for 49 h incubation and 656 ppm of initial iron concentration. The total Fe3+ content in the biomass was significantly affected by the culture temperature and degrees brix (p<0.0001). Under optimum conditions, the maximum level of Fe (III) ions in the dry cell weight of S. pastorianus was 16.82±0.65 mg/g. The results from statistical analysis showed that the model was significant (p<0.0001) and adequate.

DOI: https://doi.org/10.31276/VJSTE.62(2).48-55

Article Details

How to Cite
NHU, Pham Quynh et al. Optimization of culture conditions of iron-enriched biomass of Saccharomyces pastorianus by response surface methodology. Vietnam Journal of Science, Technology and Engineering, [S.l.], v. 62, n. 2, p. 48-55, july 2020. ISSN 2525-2461. Available at: <https://vietnamscience.vjst.vn/index.php/VJSTE/article/view/321>. Date accessed: 11 aug. 2020.
Section
PHYSICAL SCIENCES